# BATS_Feb_Aug_2012_rDNA # 16S and 18S rDNA amplicons sequenced with 454 technology # # A. Allen (JCVI) # version: 2015-09-18 # ========================= sample cruise_id season mon_year date time water_mass depth technology ------------------------- 841 AE1203 winter Feb_2012 2012-02-23 1730 mixed_layer 30 454 ========================= sample_id primer_F primer_F_seq primer_R primer_R_seq ------------------------- 841_3.0_16S (16S_F)341F CCTACGGGNGGCWGCAG (16S_R)926R CCGTCAATTCMTTTRAGT 841_0.8_16S (16S_F)341F CCTACGGGNGGCWGCAG (16S_R)926R CCGTCAATTCMTTTRAGT 841_0.1_16S (16S_F)341F CCTACGGGNGGCWGCAG (16S_R)926R CCGTCAATTCMTTTRAGT 841_3.0_18S (18S_F)EukV4F1 CCAGCASCYGCGGTAATTCC (18S_R)EukV4R1 ACTTTCGTTCTTGATYRA 841_0.8_18S (18S_F)EukV4F1 CCAGCASCYGCGGTAATTCC (18S_R)EukV4R1 ACTTTCGTTCTTGATYRA ========================= sample cruise_id season mon_year date time water_mass depth technology ------------------------- 885 AE1220 summer Aug_2012 2013-08-09 1745 mixed_layer 30 454 ========================= sample_id primer_F primer_F_seq primer_R primer_R_seq ------------------------- 885_3.0_16S (16S_F)341F CCTACGGGNGGCWGCAG (16S_R)926R CCGTCAATTCMTTTRAGT 885_0.8_16S (16S_F)341F CCTACGGGNGGCWGCAG (16S_R)926R CCGTCAATTCMTTTRAGT 885_0.1_16S (16S_F)341F CCTACGGGNGGCWGCAG (16S_R)926R CCGTCAATTCMTTTRAGT 885_3.0_18S (18S_F)EukV4F1 CCAGCASCYGCGGTAATTCC (18S_R)EukV4R1 ACTTTCGTTCTTGATYRA 885_0.8_18S (18S_F)EukV4F1 CCAGCASCYGCGGTAATTCC (18S_R)EukV4R1 ACTTTCGTTCTTGATYRA